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MOLECULAR BIOLOGY: WORKING WITH RNA

ISOLATION - RNA

1. Arabidopsis RNA Extraction
2. Schizosaccharomyces pombe Total RNA Preparation
3. Acid-Guanidinium Thiocyanate-Phenol-Chloroform RNA Purification
4. Assay of Aminocyl RNA Synthetases
5. Cesium Chloride RNA Extraction from Arabidopsis
6. Isolation of RNA from B. subtilis or E. coli
7. Isolation of RNA from Animal Tissue
8. Isolation of RNA from Small Quantities of Arabidopsis thaliana Tissue
9. Isolation of RNA from Tobacco Mosaic Virus (TMV)
10. Isolation of Total DNA and RNA from Blood Using Phenol Chloroform Extraction
11. Isolation of Total DNA or RNA from Tissue Samples Using Phenol Chloroform Extraction
12. Isolation of Total RNA from Yeast
13. Isolation of Total RNA or Poly A+ RNA from Tissue Culture Cells or Drosophila Embryos
14. Lithium Chloride RNA Extraction from Arabidopsis
15. Northern Blot Hybridization and Wash Using Ribonucleic Acid Probes (Riboprobes)
16. Poly-A+ RNA Preparation Using Proteinase K and SDS
17. Preparation of Acceptor RNA from E. coli
18. Preparation of Cytoplasmic RNA from Eukaryotic Cells
19. Preparation of Super Clean Carrier Yeast RNA
20. Preparing RNA from Reticulocyte Lysate
21. Processing of Microdissected Tissue for Molecular Analysis: DNA-based Studies, RNA-based Studies, and Protein-based Studies
22. Purity Measurement and Quantification of RNA
23. Rapid Small-Scale Isolation of Drosophila RNA
24. RNA Preparation from Twenty-Four Hour Zebrafish Embryos
25. Single Step Prokaryotic RNA Isolation
26. Single Step RNA Isolation by Acid Guanidinium Thiocyanate Phenol Chloroform Extraction
27. Total RNA Extraction from Tissue Culture Cells using Tripure or Trizol
28. Total RNA Isolation from C. elegans
29. Yeast RNA Miniprep



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